Head coach Paul Maurice told reporters that Barkov is feeling good, but he has to take warmups and see how he feels before deciding if he will be in the lineup.
The Panthers captain was forced to leave Game 2 after being on the receiving end of a hit by forward Brandon Hagel that led to him receiving a five-minute interference major.
The NHL’s department of player safety suspended Hagel for Game 3.
The Panthers lead the series 2-0.
Hagel had 35 goals and 55 assists for 90 points, all career highs, in 82 regular-season games. He also was a member of Canada’s championship team at the 4 Nations Face-Off.
Watch Game 3 of the Panthers-Lightning series on Saturday at 1 p.m. ET / 10 a.m. PT on Sportsnet or Sportsnet+.